Show simple item record

Protocol for thin section preparation of non decalcified human bone tissue

Protocolo para la preparación de secciones delgadas sin descalcificar de tejido óseo humano

dc.contributoren-US
dc.contributorProyecto de Investigación PI-40-A-463 de la Universidad Nacional de Río Negro.es-ES
dc.creatorNavarro, Tamara Giselle; Museo Provincial Carlos Ameghino. Cipolletti, Río Negro.
dc.creatorJannello, Marcos
dc.creatorJannello, Marcos
dc.creatorCerda, Ignacio A.
dc.creatorCerda, Ignacio A.
dc.creatorBéguelin, Marien
dc.creatorBéguelin, Marien
dc.creatorVázquez, Romina
dc.creatorVázquez, Romina
dc.date2018-07-01
dc.date.accessioned2018-07-05T00:25:30Z
dc.date.available2018-07-05T00:25:30Z
dc.identifierhttps://revistas.unc.edu.ar/index.php/antropologia/article/view/17007
dc.identifier.urihttp://suquia.ffyh.unc.edu.ar/handle/suquia/3170
dc.descriptionMaking thin sections from fossil and archaeological materials is usually a highly time consuming and expensive task. Also, the creation of a laboratory with necessary characteristics for the development of established protocols is also expensive. This paper presents an alternative protocol for obtaining thin sections of human bone samples from archaeological sites with the aim of applying it to microstructural analysis. The procedures for obtaining thin cuts that are detailed here are within the reach of newly formed laboratories. In the first place a femur, a tibia and a first rib n adult individual from a contemporary cemetery was measured and recorded with digital images. Fragments of 1 cm of bone were them extracted. Molds and casts of the extracted samples were prepared before thin section preparation. The obtained results were optimal for the observation of the microstructural bone features. With the appropriate modifications (set time, abrasive grading according to the hardness of the material, impregnation, etc.) this protocol could be useful for several kinds of samples (extant, archaeological and even fossil ones).en-US
dc.descriptionLa obtención de cortes delgados de restos óseos correspondiente tanto a materiales fósiles como arqueológicos suele ser una tarea poco sencilla, lo cual conlleva generalmente un elevado costo y tiempos prolongados. Por otra parte, la formación de un laboratorio con las características necesarias para la realización de protocolos ya establecidos es sumamente costosa. En este trabajo se presenta un protocolo alternativo para la obtención de cortes delgados de muestras óseas humanas de sitios arqueológicos con el objetivo de aplicarlo al análisis microestructural. Los procedimientos de obtención de cortes delgados que aquí se detallan están al alcance de laboratorios de reciente formación. En primer lugar, se midió y se registró con imágenes digitales un fémur, una tibia y una primera costilla de un individuo adulto procedente de un cementerio contemporáneo. Luego se extrajeron fragmentos de 1 cm de hueso. Con el material se confeccionaron réplicas y por último se obtuvieron los cortes delgados. Los resultados obtenidos fueron óptimos para la observación de las características microestructurales del hueso. Con los ajustes adecuados (tiempo de fraguado, granulometría del abrasivo de acuerdo a la dureza del material, impregnación, etc.) este protocolo podría ser utilizado para diversos tipos de muestras actuales, arqueológicas e incluso fósiles.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherFacultad de Filosofía y Humanidades. Museo de Antropologíaes-ES
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/view/17007/20153
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4316
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4317
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4318
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4319
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4320
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4321
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4322
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4323
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4324
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4325
dc.relationhttps://revistas.unc.edu.ar/index.php/antropologia/article/downloadSuppFile/17007/4326
dc.relation/*ref*/ssis, S., A. Santos y A. Keenleyside. 2016. Paleohistology and the study of human remains: past, present and future approaches. Revista Argentina de Antropología Biológica, 18(2), 1–17.
dc.relation/*ref*/Buikstra, JE., DH. Ubelaker. 1994. Standards for data collection from human skeletal remains. Proceedings of a seminar at the Field Museum of Natural History. Organized by J. Haas. Arkansas Archaeological Survey Research Series No. 44
dc.relation/*ref*/Cambra-Moo, O., CN. Meneses, MA. Rodríguez Barbero, O. García Gil, J. Rascón Pérez, S. Rello-Varona, MC. Martín y A. González Martín. 2012. Mapping human long bone compartmentalisation during ontogeny: A new methodological approach. Journal of Structural Biology 178: 338-349.
dc.relation/*ref*/Chinsamy, A., MA. Raath. 1992. Preparation of fossil bone for histological examination. Palaeontologia Africana 29: 39-44.
dc.relation/*ref*/Chinsamy Turan, A. 2005. The Microstructure of Dinosaur Bone. Johns Hopkins University Press, Baltimore and London.
dc.relation/*ref*/Desántolo B., V. Bernal. 2016. Los estudios de histología ósea en antropología biológica. Revista Argentina de Antropología Biológica, 18(2), 2015–2017.
dc.relation/*ref*/Desántolo, B. 2012. “Validación metodológica para la estimación de edad en restos óseos humanos adultos: análisis histomorfométricos”. Facultad de ciencias médicas, Universidad Nacional de La Plata, Argentina, 187.Tesis Doctoral. SEDICI (Repositorio Institucional de la UNLP) http://hdl.handle.net/10915/51327.
dc.relation/*ref*/Comparison of 3D Landmark and 3D Dense Cloud Approaches to Hominin Mandible Morphometrics Using Structure-From-Motion. Archaeometry 59 (1): 191-203
dc.relation/*ref*/García-Martínez, D., García Gil, O., Cambra-Moo, O., Canillas, M., Rodríguez, MA., Bastir, M. y Gonzalez Martín, A. 2017. External and internal ontogenetic changes in the first rib. American Journal Of Physical Anthropology 1-13
dc.relation/*ref*/Gosman, JH. y Ketcham, RA. 2009. Patterns in Ontogeny of Human Trabecular Bone From SunWatch Village in the Prehistoric Ohio Valley: General Features of Microarchitectural Change. American Journal Of Physical Anthropology 138:318–332
dc.relation/*ref*/Hollund, H., M. Jans, M. Collins, H. Kars, I. Joosten y S. Kars. 2012. What happened here? Bone histology as a tool in decoding the postmortem histories of archaeological bone from Castricum The Netherlands. International Journal of Osteoarchaeology 22:537-548. doi:10.1002/oa.1273
dc.relation/*ref*/Jans, MM., H. Kars. 2002. In situ preservation of archaeological Bone: a histological study within a multidisciplinary approach. Archaeometry, 44 (3): 343–352.
dc.relation/*ref*/Katz, D., M. Friess. 2014. Technical Note: 3D From Standard Digital Photography of Human Crania-A Preliminary Assessment. American Journal of Physical Anthropology 154 (1): 152-158.
dc.relation/*ref*/Lander, SL., D.Brits y M. Hosie. 2014. The effects of freezing, boiling and degreasing on the microstructure of bone. HOMO-Journal of comparative human biology 65, 131-142.
dc.relation/*ref*/Maggiano IS., CM. Maggiano, JG. Clement, CDL. Thomas, Y. Carter, DML. Cooper. 2016. Three-dimensional reconstruction of haversian systems in human cortical bone using synchrotron radiation-based micro-CT: morphology and quantification of branching and transverse connections across age. Journal of Anatomy 228(5): 719-732.
dc.relation/*ref*/Nacarino Meneses, C., O. Cambra-Moo, MA. Rodríguez Barbero y A. González Martín. 2012. Aportaciones de la paleohistología humana al estudio de los biomateriales. Boletín de la sociedad española de cerámica y vidrio 51 (6): 313-320.
dc.relation/*ref*/Padian K., ET. Lamm. 2015. Bone histology of fossil tetrapods. Advancing methods, analysis, and Interpretation. University of California Press. 285 pp
dc.relation/*ref*/Robling AG., SD. Stout. 2008. Histomorphometry of human cortical bone: applications to age estimation. En Katzenberg MA y Saunders SR . Biological anthropology of the human Skeleton. pp 149-182. New York: Willey Liss. Inc.
dc.relation/*ref*/Rösing, FW. M.Graw, B.Marré, S.Ritz-Timme, MA. Rothschild, K.Rötzscher, A.Schmeling, I. Schröder y G.Geserick. 2007. Recommendations for the forensic diagnosis of sex and age from skeletons. HOMO- Journal of comparative human biology. 58. 75-89.
dc.relation/*ref*/Schultz, M. 2001. Paleohistopathology of bone: a new approach to the study of ancient diseases. Yearbook of Physical Anthropology 44, 106–147
dc.relation/*ref*/Singh IJ., DL. Gunberg. 1970. Estimation of age at death in the human males from quantitative histology of bone fragment. American Journal of Physical Anthropology. 33 (3):373-392
dc.relation/*ref*/Ubelaker, DH. 1998. The evolving role of the microscope in Forensic Anthropology. Reichs KJ, editor. Forensic osteology: advances in the identification of human remains. Springfield: Charles C. Thomas. P. 514-532.
dc.rightsCopyright (c) 2018 Tamara Giselle Navarro; Marcos Jannello; Ignacio A. Cerda; Marien Béguelin; Romina Vázquezes-ES
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceRevista del Museo de Antropología; Vol 11 (2018) NÚMERO 1; 121-126es-ES
dc.source1852-4826
dc.source1852-060X
dc.subjecten-US
dc.subjectProtocol; Thin section; Human bone remains; Histologyen-US
dc.subjectAntropología Biológica; Bioarqueología.es-ES
dc.subjectProtocolo; Corte delgado; Restos óseos humanos; Histologíaes-ES
dc.titleProtocol for thin section preparation of non decalcified human bone tissueen-US
dc.titleProtocolo para la preparación de secciones delgadas sin descalcificar de tejido óseo humanoes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typees-ES
dc.typeInvestigación aplicada;Histología.es-ES
dc.typeen-US
dc.coverageen-US
dc.coverageen-US
dc.coverageen-US
dc.coverageSudaméricaes-ES
dc.coverageRestos Óseos humanos actuales.es-ES
dc.coverageRestos Óseos Humanoses-ES


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record